Comparison of vanA gene mRNA levels between vancomycin-resistant Enterococci presenting the VanA or VanB phenotype with identical Tn1546-like elements
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چکیده
منابع مشابه
Surveillance of Vancomycin-resistant Enterococci colonization with GeneXpert vanA/vanB test and culture method
Objectives: Early detection of vancomycin resistant enterococci (VRE) colonization in hospitalized patients plays an important role in controlling enterococcal infections. For this purpose, the detection of VRE from rectal swab specimens can be performed with various methods. Methods: In this study, VRE colonization in the rectal swab samples of patients from intensive care units, burn unit, an...
متن کاملComparison of the Roche LightCycler vanA/vanB detection assay and culture for detection of vancomycin-resistant enterococci from perianal swabs.
We compared the performance characteristics of a real-time PCR method, the LightCycler vanA/vanB detection assay (Roche Diagnostics Corporation, Indianapolis, Ind.) to that of Enterococcosel agar (BBL, Sparks, Md.) for direct detection of vancomycin-resistant enterococci (VRE) from 894 perianal stool swabs. For 421 of 894 swabs, the result for LightCycler PCR was compared to an Enterococcosel p...
متن کاملComparison of PCR and culture for screening of vancomycin-resistant Enterococci: highly disparate results for vanA and vanB.
We compared PCR to conventional culture for the detection of vancomycin-resistant enterococci (VRE) in 30,835 rectal samples over a 3-year period. The positive and negative predictive values of vanB PCR were 1.42% and 99.9%, respectively. A positive vanB result by PCR is poorly predictive and necessitates culture for differentiation of VRE-positive and -negative individuals.
متن کاملDistribution of insertion sequences associated with Tn1546 and clonal diversity of vancomycin-resistant enterococci isolated from patients in Tehran, Iran
BACKGROUND AND OBJECTIVES Infection with vancomycin-resistant enterococci (VRE) has caused a therapeutic problem. VanA and VanB resistant types are the predominant phenotypes among vancomycin resistant enetrococci. Transposon 1546 (Tn1546) harboring the vanA gene cluster, plays an important role in the horizontal transfer of vanA gene. In this study, we examined the phenotypic and genotypic div...
متن کاملDetection of both vanA & vanB genes in vanA phenotypes of Enterococci by Taq Man RT-PCR
Twenty seven isolates of vancomycin resistant Enterococci based on the disk diffusion and E- test have been screened; being found eight (0.3%) clinical isolates of vanA & vanB through Taq Man Real Time PCR assay. This study shows the presence of both vanA & vanB genotypes in vanA phenotypes clinical isolates in the three hospitals in Iran.
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ژورنال
عنوان ژورنال: Journal of Microbiology, Immunology and Infection
سال: 2016
ISSN: 1684-1182
DOI: 10.1016/j.jmii.2014.09.003